Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSALELPAKILEW-IEKAAGSPVLKADRIPGGGTRQGWLVDVAAAAAGDGSGKLF-LRYSPERLPDRGAFHRLATEAEV-LRALRGAGVAVPA-VCAVH---PVEEAVLL-ERVPGETWFYRIRDP-DEQVRVAQDFI-RSLAKV-HRLDPADLGVPGLGPVRSAREHALERIAEIRRRGTAPDGSIDPLVR-LSA-DWLESHVPDYD-GPVVLVQGDTGPGNF-LY-QDGRVTAVLDWELCHFGDP-MDDIAWL-SLRTVQDTFTHLPDRLAEYEKLSGHAIDVDRVRYYRVFAETTMATLTPNEGARAPAAEDGSGGADKDVGNHLLYAQLHRRLWLEALNAVMGLGLKKPVMPEPVDSSDWQPLYGDVLAMLRTITPRMGDALAVQWSKGIARL-VRYLRELDAC-GRAGNELERSDIASLLGFTPLS-LP-AGRDALARANAAGRVSDVDFTRYLWNRVMRDDHLMRHASGALHSRTWPPLTNPS 3DXP Chain:A ((20-359)) ---RFDTEALEAWMRQHVEGFAGPLSVEQFKGQSNPTFKLVT-------PGQTYVMRAKP----------GAIEREYRVMDA-LAGTDVPVAKMYALCEDESVIGRAFYIMEFVSGRVLWDQSLPGMSPAERTAIYDEMNRVIAAMHTVDYQAIGLGDYGKPGNYFQRQIERWTKQYKLSET-----ESIPAMDSLMDWLPQHIPQEDADLTSIVHGDYRLDNLMFHPTEPRVLAVLDWELSTLGHPMGDFGYHCMSWHIAPGQFRGIAGLDHAALGIP------------------------------------------------------------------------------------------DEASYRKLYEQRTGRPITGDWNFYLAFSMFRIAGILQGIMKRVVDGTASSAQALDAGKRARPMAEMG------------------------------WEYAKKAKQ--------------

General information: TITO was launched using: RESULT: Template: 3DXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1371 13597 9.92 44.29 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 9.92 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_3DXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxp-query.scw PDB file : Tito_Scwrl_3DXP.pdb: