Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSRTKNLLSHEDIQRLVKVNFGEEYEHKIGEIKELKGGMFNAIYLIQRVDEKNGIVLKVGVAPGRDLLTYEQDIMPTEVACYQLIKEK--TSIPIPEILKYDFSKKYIDSNYFFMEELKGVPLSKA-SKLMNKPSLDKIKSKLAEYLVQLHKIEGNYFGYFTDEKKYQYDTWKNAFLSMFGQLLEDCKFHKVNIPYERIEQALKYNIQFLEEIKKPSLVMFDCHEGNVFVNKRNGEYEIQGIIDFERAFWGDPAADFPAAFIMS---DDIRKEEAFLASYFEKSDTKKEYTRDDEIRFQMYRMYILTVMAGETFRYGPLYAKMQREWAKSGILKCLEILED 5IQI Chain:C ((9-302)) -------ATNVKAMKYLIEHYFDNFK---VDSIEIIGSGYDSVAYLVN---N--EYIFKTKFSTN------KKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYIS---DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID--NKQNVL-EEYILLRETIYNDLT----DIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGN---NRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNID------IEKAKEYQDIVEEYYPIETIVYGIKNIKQ---EFIENGRKEIYKRT-----

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1286 -17175 -13.36 -59.64 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -13.36 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: