Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QDLRRPLLCDVYIEYPSGQVFDQALGHDIVPAGETAEISGFKCKT-----VRGTCV---------------------- 5TC1 Chain:M ((1-393)) MRAFSTLDRENETFVYRSNWTPGRFNSTGAKTKQWHYPSPYSRGALSVTSIFSLDARSCYSLFPVSQNLTYIEVPQNVANRASTEVLQKVTQGNFNLGVALAEARSTASQLATQTIALVKAYTAARRGNWRQALRYLALNEDRKFRSKHVAGRWLELQFGWLPLMSDIQGAYEMLTKVHLQEFLPMRAVRQVGTNIKLDGQTTCNISRRIVIWFYINDARLAWLSSLGILNPLGIVWEKVPFSFVVDWLLPVGNMLEGLTAPVGCSYMSGTVTDVITGESIIQGTAKAQISAMHRGVQSVWPTTGAYVKSPFSMVHTLDALALIRQRLSR

General information: TITO was launched using: RESULT: Template: 5TC1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 102 -5317 -52.13 -104.25 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain M : 0.51 3D Compatibility (PKB) : -52.13 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_5TC1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5tc1-query.scw PDB file : Tito_Scwrl_5TC1.pdb: