Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREPSSLQERDPDPFSEQMIALMVAAAGIEC-EVLDAEPLRTGSA-TTYRVDVRDQEGDRRTVVFKAAVEGDATGLRREPALLRYVADHTSIPVPDVYGSHLQPDDLPAPSFVMEHVEGGSPTQASARENGDTGWPDGILDDIVRQAGRHLAALHDCGPVSGFGPITVDDGTLTVTDPASSWQSWLWELAAEIEGDTRLDGLASTVQSVLDSEIPELPDVDPVLAHYDYQPRNVVVDEGAGRVRAVLDWGGARSVHDEFELAVTEQYCSGWAPL--------GSDKRQRVRASLYEGYRDRRSLATDEAFRRRRRLYLLLSTVASLSWAPYWSGPRAERFVASQRSLVESLLDQGP 3DXP Chain:A ((20-318)) -------------RFDTEALEAWMRQHVEGFAGPLSVEQFKG-QSNPTFKLVTP--GQ---TYVMRAKPG----AIEREYRVMDALAG-TDVPVAKMYALCEDESVIGRAFYIMEFVSGRVLWDQSL-----PGMSPAERTAIYDEMNRVIAAMHTV-DYQAIGLGDYGKPGNYFQRQIERWTKQYKLS------ETESIPAMDSLMDWLPQHIPQEDADLTSIVHGDYRLDNLMFHPTEPRVLAVLDWELSTLGHPMGDFGYHCMSWHIAPGQFRGIAGLDHAALGIPDEASYRKLYEQRTGRPIT----GDWNFYLAFSMFRIAGI----------------------------

General information: TITO was launched using: RESULT: Template: 3DXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1218 2 0.00 0.01 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 0.00 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_3DXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxp-query.scw PDB file : Tito_Scwrl_3DXP.pdb: