Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GADTRLVPGCLVKLYRPPKYIGCFDRSILVGAKCIPVQTT-------WKI-KGHDISVWNGCSTVKGINLPKGWFVKGE 5CHC Chain:B ((20-65)) --------------------------------KCIGCQTCTVACKKLWTTGPGQDFMYWRNVETAPGLGYPRNWQTKG-

General information: TITO was launched using: RESULT: Template: 5CHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 66 -633 -9.59 -16.66 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -9.59 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.175

(partial model without unconserved sides chains): PDB file : Tito_5CHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5chc-query.scw PDB file : Tito_Scwrl_5CHC.pdb: