Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQ-HASKQITADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWLYFAGNLASGGAAGATSLCFVYPLDFARTRLAADV--GKAGAEREFRGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVISWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGGKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYT 4C9J Chain:A ((25-313)) -----TNFAINFLMGGVSAAIAKTAASPIERVKILIQNQDEMIKQGTLDKKYSGIVDCFKRTAKQEGLISFWRGNTANVIRYFPTQALNFAFKDKIKLMF--GFKKEEGYGKWFAGNLASGGAAGALSLLFVYSLDFARTRLAADAKS-----ARQFNGLTDVYKKTLKSDGIAGLYRGFMPSVVGIVVYRGLYFGMFDSLK--------------FLLGWVVTTGASTCSYPLDTVRRRMMMTSGQA---VKYNGAIDCLKKIVASEGVGSLFKGCGANILRSVAGAGVISMYDQLQ---

General information: TITO was launched using: RESULT: Template: 4C9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 -11072 -10.31 -41.62 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -10.31 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_4C9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4c9j-query.scw PDB file : Tito_Scwrl_4C9J.pdb: