Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSDEASRFLAELGIADDSVTPITGGWASWTFATGSGRILRVARNPEIDAAHRRESRLLPELAEA-VSFAVPAPTH----FGVHKGM-----TYMVYPRLPGRGLEPGDRVRAVAPMLAELHAFPVDRAAELLGCAVTAEAWRDDYLEIGKWVEG--EVLPVLDGELRDRVRREYDATLPD--LAAISPALIHRDLGCEHILTDPETGMPTGIIDFETATVGDPAIDYVGLLVT----FGEQVTRELIAA---SGSEISWRLVRFYHWLGAAHAVKYGLAERDDAIVADGIEGLRRRLPG 3HAM Chain:A ((24-289)) ----------------------LSSGDDSDTFLCNEQYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVYQSDRFNIMKYIKGERITYEQYHKLSEK--EKDALAYDEATFLKELHSIEIDCSVSLFSDALVNK--KDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLVHNDFSANNMIF--RNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKGVSEL------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1027 6174 6.01 25.20 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 6.01 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: