Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELNASEFRSLITSVFPELTASVFKLAAKSWDSLAVDVDDTLIFKFPRHPAAERALVKEAGVLDIVRPSLSMAVPDMHIHDGPP------IFSSHAKLDGEHLIAEDYDALGQGVRRHLADDLARFYAELHVLDADRLRQAGVGAIQPWQSPDAVRTKAL---PLLPPDIGR---FAEAVIAD---FEALPPDPHGTVFGFFDGHGWNMAFDHAQGRLNGIYDFADSGFGPLHQEFIYSNFISADLTARIVSAYEMLTGRKLDRRRIAILTGFHRLSELAELADDPAHVDLMVQNVATWAAVAAHVG 5C4L Chain:A ((33-251)) ------------IEQLYPDFTINTIQISGKGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEV-VFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHN------DFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFI----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5C4L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 683 -8698 -12.73 -42.63 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -12.73 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.208

(partial model without unconserved sides chains): PDB file : Tito_5C4L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5c4l-query.scw PDB file : Tito_Scwrl_5C4L.pdb: