Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPAKWRETIDPFELDYHHFRLTEILGYPHAGNDVFHAKGIYQQQETEVYIKV--ARQQGA--DIKREIDTILKLNLEL-APEIIDYDQNREQFVVTIARQGERLSMLLNQNKDEKSIDYLFE-YGQTLAILHQ---TQGNFENVKDRRFFHIP--LEEHFRELRLE------------EVYHYLVKHKPERETRCFCHGDFHYANILWKEKHISAILDFELSGMGNREFDIAWALIVRPGQRFLLTEEEIREFLRGYASEGTFQEDLVRYYMVLIYSFFYKMGETEYQDFVRSFLQQNIR 1J7I Chain:A ((41-225)) ------------------------------------------------LYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCS--EEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEEL-VFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCV-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1J7I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 457 16661 36.46 102.84 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 36.46 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.207

(partial model without unconserved sides chains): PDB file : Tito_1J7I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j7i-query.scw PDB file : Tito_Scwrl_1J7I.pdb: