Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSYEFN-VTQLRDLPFGDKNIKIITEKKGGWDSRFFILDGKVAVKVPRNITVQHSIIKEKIVTDLLRKELPLPIPDMNLAEIDSIDGNKIIIVIYEMLPGIELQNIHII---GENKIKTSKSLGLFLQKLHNI-----PDELAQILRSGISNVNNERNETDLELLNSVLEYFEEMRNKTNVSSYIEIMRTSMGSLKQMGTKIVPC--HGDFMSNNILYNEIEGKISGIIDWGDFSFRNPIYDFAGL---TYEFGEEFLDSMLAAYASNTSPDFREKIVKLSRFVPFITVYFSRHMKKKIRFEKKYIKIELLESGRNQILHVR 5C4K Chain:A ((22-317)) -RTYTFDQVEKAIEQLYPDFTINTIQISGKGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPS-ETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKI----NEDNKKIKKLLSRELK-GPQMKKVDDF--YRDILEN------EIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSF----------EKIIYGKEYGYMDWYEEGLNEIRS--

General information: TITO was launched using: RESULT: Template: 5C4K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1231 43690 35.49 154.93 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 35.49 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_5C4K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5c4k-query.scw PDB file : Tito_Scwrl_5C4K.pdb: