Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKLKNLVLNTDQVANAILKNWNHDENSLNFWRGSANYVYFFTLKGDR-YWLRFSRKDENSLEQIKAEIEFLLYLKENNFSAVYPIISNNHRYIEVVEDEGETYYAVVFNKAEGVNLEIEDMTEAEFECWGKSLASLHRLSK----TFRPKEHV-------RNSWKQVLEVTSDILSDFPNEQNAKDEL--KKVEKWLKSLPVTNENYGLIHYDFELDNVFFNKQSRLFKAIDFDSSMYHWYVMDIACTLGDLNELDSPKAEDGLKHFLKGYRSINGIEEEFIDLLPKFERFDNLISFAKLLRSLQDSSFPSEPEWLKRLRPRLLKKCDEYRKGFEKAW 1ZYL Chain:A ((10-297)) -------TLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLN-----HQGFYFAVFPSVGGRQFEADNIDQ--MEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDATLIPSGLKAAF---LKATDELIAAVTAHWREDFTVLR-----LHGDCHAGNILW-RDGPMF--VDLDDARNGPAVQDLWMLLNG----DKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWL----------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1245 -9789 -7.86 -35.73 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -7.86 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: