Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVEQNEFLSQVQLQILAEQAIKQYPSDY-QGQVKLLCQ-SENATYQVSTGSARYALRIHRPNYH-SKLDI-QSELEWLDALNASGIQVPVAIADQSGERVITLKLSNDIYRYAVLFNWVEGDMPTVEV-DPTAFEQLGQITAKLHAHSKTWQAPENFQRIVWNHETMVGADGHWGNWKNA---PHLR--PQDHGVIEEAIAQISKDLNFFGKTQERYGLIHADLRLTNLLLQQ---ERIGVIDFDDCGMSWFMHDLAAAISFNEH----Y-------ANAPHWVDYWLKGYER--VGHIQSEEYEMIPTFIMQRRIQMMAWNGSHAQTEMAQSLGDQWSNETVRLCKKYLNGQMPVGI 4OCV Chain:A ((24-326)) -------------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEI-----DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTE-TIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMR-------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1209 -24254 -20.06 -87.56 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -20.06 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: