Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVYTPLSLDEVRTFAAPYGL--EVLELNPIQGGIQNTNYFLVDVNRKQYVLTVFEELD---AQGA-GELIPVLEQLGTHDVPVAVPLKH-SGQAVHFIAGKPAQIAPRLMGHHPMQ--TTVAQVAAIADAQAKLHVALQDFPLEREYR--R----DHQYWTGVAEQLKPN-MT--QDDQTLLEQVYQAFN---AKTA--QYSNRPTGFIHSDLFRDNTLFEG--EQLQGILDFYELNQDELLFDIAITINDFCTEY-------PAAHLNSDKVGAYLEAYQK--VRALTSDELECLDVFLAMAACRFWSMRLQVAQKNKEEGRTGDDILQKDPQEMRAMMQDRLSHVKA 4OCV Chain:A ((24-319)) -------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTT-DGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLL----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 12019 11.19 45.87 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 11.19 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: