Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAG-HDFFVKCDEREL-LPGFTAEADQLELLSRSK-TVTVPKVWAVGADRDYSFLVMDYLPPRPLDA-------------------HSAFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAAEKGIAFGNIDAIVEHIQQRLAS---HQPQPSLLHGDLWSGNCALGP-DGPY-IFDP-ACYWGDRECDLAMLPL--HTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIAQQSLDRLLAA 5UXA Chain:A ((7-257)) ---QVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT----EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLV---V--HTPEEARMSMKQRMDAV-----RAKFG----V---GENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAFGEEGLEALILAYKEIGGY-----------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 829 -17762 -21.43 -80.37 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -21.43 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: