Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVYTKLSDEYIKNILKSYSFKKVDRIEEISEGILNTNYFVEGDKGKFIFRILEGE-RDYTEEVKELEFLEYLNL-NGFPCPTAIKNDLGENYTFID----GKMTSVFTFIEGEKVKS--INENNIKEIGQKLGKMHNLLKDRDIKRN-R--KIDMQYFYDIISK---A-D-------LRGILKDDYDFIMKYYERASKIDY-SNLPFGIIHNDIFPDNVFMQEG---EISGIIDFNDCLRGPLILDLAIVISFWIRNRGFSDEVENRLTEVFLNAYESERKITEEEKELMNEALIRIALTFIFLRVNKFHVEDNSSVNMEFKNYKDLLPLLRYF 6EF6 Chain:A ((27-308)) -------DVTIAQQALTHYDVSDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLE---EY-GSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD-----PRLGEWQESWVAGYRSRRELPAADEAMLPSFVF---------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1113 -7297 -6.56 -28.84 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -6.56 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: