Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MQLTHESQQLTTPKENGWCGPLPRLVAS------LSSLVLPLPRTESYEFA---GCAVNNRFDPR--------------QGAFRDLPSS--PPIPL-------------------AVSLPLNNP-PANFRSSAASMPPSPVRRPAVPP-------------------RQPFTSRTNALEGFPSPHPSSTPSKRRLAPSATLPPPSYAREHGIFLG-----------------RRRPKDCEGVGQFVKRVHWEGK----VMVVKYGRSVRQAEVDVMKMVRERTHVPVPEVFGTAFDEAAGELFIYQ------EFIEGTTLTAAWPTLSH-------------PDLSRIKSDFSRYVHELGALALPPD---------------------ASLGSCDASSSYRLSEPCHAVFAQKPEPKVQSVE-DFTRWIRFEISRRSRDLANSRKDFDLERLFRDGD--VGLVHADLHGEN-----ILVKNGRIAAILDWEL---AAWMPPLVEALSLVEY--SRMFDEDAGAPIDALIDALGLEDATRD--VLDGLCTALRAWPDEKWEEVS----------------------------------------- 5L8S Chain:A ((1-604)) MINFPKPTVEQFFRTYTITNFAVSSDE------KRLVFNANLNGKMNLWAMDLPDTYPYLFAHRDESCNFIKFDPENRYVLAGFDKDGDENYQIYAIPNEGGLPHPLITGDASEKYYFSHLSADGKCVYYETSKENPSFLNTRIRNLETGEDRLLNVGEVSTTELAAVSENEESFVYLRAFANTYIVGFVKMGEETFNITPDPEKVHVAME---PVFTDNETIYFATDYDSDEMYLAKFDLTSKEFSKVLAFDGESIQSVKWDKDNKAFYLITVKGVTDILYRYDVATDKVEECSLPVDIIEQIQVAKS-GNLYILGRSATVPHNVYQSSNGVEWKQLTNNRVLGLSPEDMVEPDIVSYTSFDGMEIEALLFKAKPENDNGYTIFWPHGGPQSAERKMFRSMFQCFINRGYTIFAPNFRGSTGYGSAFTKLVELDWGEGPRLDCIAGIEWLFESGF-TDRNKLFLVGGSYGGYMALLLHGRHSDYFRAVVDIFGPSDLFTFINSVPPHWKPIMERWLGDPERDKERFIKDSPVTYLDGMVKPMLVIQGAKDPRVVKEESDQIVAKLKEKGRDVEYLVLEDEGHGFSKKENEIKVYSLMLAFLEKHQALEHHHHHH

General information: TITO was launched using: RESULT: Template: 5L8S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2255 61451 27.25 149.15 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 27.25 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_5L8S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5l8s-query.scw PDB file : Tito_Scwrl_5L8S.pdb: