Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAADPTVPYAGLSPERILEALESVGLRPDGRLLAL-NSYENRVYQVGMEESAPLVAKFYRPGRWTREAILEEHRFVQQLADA-EIPAVPPLAFAAEFVGQLADDRGSCTLPEYGGFAFAVFPRCPGRVPAL-----DQPDTLQWMGRFLGRIHALGTLEPYRHR-LTLDIDSFGHAPRDWLLAHDMIPPELLPAWRSVVDQALEAVQACHARAGNVNLLRLHGDCHAGNVLWIDEADAPRYGAGALPGPHFVDFDDSCMGPAVQDLWMLLSGDRAAMTRQLGDILAGYEDFCSFDP-RELHLVEALRTLRLIHYSGWLARRWDDPAFKAAFPWFDTPRYWQDRILELREQVALMQEPPLVA 5IGI Chain:A ((5-279)) ---------TTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRA-EVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELV-----------AYP-MLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDR---VRREFV-VNDKRLHRWQRWLDDDSS--WPDFSVVVHGDLYVGHVLIDNTE--------R--VSGMIDWSEARVDDPAIDMAAHLMVF---GEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYAL---------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1202 -55695 -46.34 -210.17 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -46.34 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: