Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPTPSPDTILFASHTTATAASVAQWVERHYDLP-VQQCYLIRRNLNDNYAVRAADGSRYVARLCAIRPRGPFNVDFEVALLAHLEA-QGVGVASPVLAANGATSVALPF--PEGPRVLVLFRHCEGSVPD----TPEDLHLTGATLAQIHSAAQSYTGPA--SRYVLDGHHLAGRTLDYLAAHPELA---PSVLDAYRELIHRLLGELANV-EDTLTRVMCHGDTHGFNNHVYSD---AEGARRAAFFDFDDAGPGFLAYDLCVWLWSNLPRKAPMEPDEALLQRWQQYLAGYRAGGGHVTDADLAALPLFVQLRHLWNMGEGVARIHHWGANMMSVDWIKKQLEVMAAWARLDLPA 6EF6 Chain:A ((27-323)) -------------------DVTIAQQALTHYDVSDNASLRLLNLSENATYLVE-DGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD--VPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDT-PRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQR----TITVIDFDDCGFGWYFYDFGTAVSFIEHD-------PRLGEWQESWVAGYRSR-RELPAADEAMLPSFVFLRRLLLLAWMGSHTH-----------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1301 -39342 -30.24 -142.03 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -30.24 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: