Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTRRPEIPADLRQW-IAEMTGTDVVEVRQVPGGASRQAWFVDAGTGARSRALFLRYD-PREADPGSAFHPLQVEAEI-MAELHRHGVTVPQ-VIAAHPTL---QAVLL-ERVGGDTWFRLIQDP-DEQVRTARDFI-GKLAAL-HRIDAQHLTIPSLGPAGPVAGHVRAEIAAMRARLSRYGKPAPLLA-FCI-DWLDRHVPDYD-GPTVMVQGDTGPGNF-MY-SGGVVTAVVDWELAHFGDP-MDDIAWLS-L--------------RTVQDTFTDFPARLAEYEQLSGHRIDDDRVWYYRLFAE-TRLASISPGS-IDTRASVPPASPDAGNSLIYGM-LH-RRLLVEALAHVVGIPEVDVELPPEGDADAPSEHSSVYEAAASAMSGAAARSTDALALRYVKGAARLVKYLAEVDRIGAVVDAQETAELTAVLGWAPRSVSQGRAALAELAGRGDLTDRDYVTQLWRGIKHDDYLTRTASGALARRTWPPLRHPVPPPSVEQGDRDRWVSPSPKTTVS 3DXP Chain:A ((20-359)) -----RFDTEALEAWMRQHVEGFAGPLSVEQFKGQSNPTFKLVTPGQ--------TYVMRAKP------GAIEREYRVMDA-LAGTDVPVAKMYALCEDESVIGRAFYIMEFVSGRVLWDQSLPGMSPAERTAIYDEMNRVIAAMHTVDYQAIGLGDYGKPG---NYFQRQIERWTKQYKLS-ETESIPAMDSLMDWLPQHIPQEDADLTSIVHGDYRLDNLMFHPTEPRVLAVLDWELSTLGHPMG-DFGYHCMSWHIAPGQFRGIAGLDHAALGIPDEASYRKLYEQRTGRPITGD--WNFYLAFSMFRIAGILQGIMKRVVDGTASSAQALDAGKRARPMAEMGWEYAKKAKQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1336 15155 11.34 51.72 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 11.34 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_3DXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxp-query.scw PDB file : Tito_Scwrl_3DXP.pdb: