Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYQWEPQQTLEPPLALELIREQFPDLSPVQIRVLGAGWDNTAFVIN----EDLIFRFPRREIAVPLLEAEWCILPKLAPR-LPLPIPIPKWRGSPASRFPWPFIGYKMLPGFTACYANL----------SEREREALAQPIARFLSHLHATPRSIISGCQIPGDN--QGRIDWKQLMPKIRKNIEELSLLSLLEKRNELEALLESLQNLRPPMASIVVHGDFYVRHILVDEIHHFAGVIDWGDIHLGDPAIDLSVAHSFLPFCAHEKFREAYGEIST----DTWSLALLRAIYSSTLLVLYGHHSKDPVILREGLRSLKIMVGK 5UXA Chain:A ((3-299)) ----------KDIKQVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT-------EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRAKFGV----GENLWNRWQAWLNDD---DMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAFGEEGLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEFALVSGIEEYEQMAKEALEV----

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1211 -29298 -24.19 -106.15 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -24.19 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: