Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVFTPVSLDDLTTWITQFDLG-RALAIKGISSGIENSNFFITTERGEYVLTVFEKLT---F-EQLPFYLNLMRHLAEGGVSVPAPVANRNGSIINALHGKPASIVTKLEGHCQLA--PQPVHCAAVGAMLACMHLAAEDFEIRQPNL--RGLE----WWNEVTPIVMP----YLS--DSNEQLLATEIISQNAFADS-D--VFRELPNGPIHADLFRNNVMFEG--EQLTGFFDFYFAGCDTWLFDVAVTVNDWCIDDDS-------GELDQARVRAMLDAYHA--IRPFTDAEQGAWQTMLRASALRFWLSRLYDFHMPRDAEMLTPHDPAHFERILRLRIAQTAPALFTTAG 4WH3 Chain:A ((4-300)) -------SNEVLFGIASHFALEGAVTGIEPYGDGHINTTYLVTTDGPRYILQQMNTSIFPDTVNLMRNVELVTSTLKAQGKETLDIVPTTSGATWAEIDGGAWRVYKFIEHTVSYNLVPNPDVFREAGSAFGDFQNFLSEFDASQLTETIAHFHDTPHRFEDFKAALAADKLGRAAACQPEIDFYLSHAD-Q--YAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDEKDLSKVHFSTELFRAYTEGFVGELRGSITAREAELLPFSGNLLT------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4WH3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1082 -10709 -9.90 -40.56 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -9.90 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_4WH3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4wh3-query.scw PDB file : Tito_Scwrl_4WH3.pdb: