Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MEMRGGDLEAAALALWPDLARQMG----------EEAGLWRAAPLA--------RREDARVARVLLRLDGPEGRRLVLKHQARPVVPDKFADGIAAHVAVQEVWPEGLPVLQAVDLERQACVMEYLPARPLSVLLEGAPLETQAALLRRAGAWMDGF-----HRALHGEARVFQPKFTIHFLRSVMGELRAGER-RVAEPERFLVCAEALCAMQPDFEGQETVTAQTHGDLHQRNLVLDETRCWGVDFAGGRVVPVGHDIARLLTD---YAILHAPKEAIPKGQVL---------PPQAQSAFFEGYGLVGPGD-----------------PSVQLLLRNRVLAEWWGLPAREAERS--------------------RAQARRFGRLMALVDRVFG---------------------------------------- 2IQH Chain:A ((21-489)) NATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDTGGPIYRRVDGKWRRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINRRTRIAYERMCNILKGKFQTAAQRTMVDQVRESRNPGNAEFEDLIFLARSALILRGSVAHKSCLPACVYGSAVAS----------GYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTKVVPRGKLSTRGVQIASNENMETMESSTLELRSR----YWAIRTRSGGNTSSGQISIQPTFSVQRNLPFDRPTIMAASDMRTEIIRLMESARPEDVSFQGRGVFELSDEKATSPIVPSFDMSNEGSYFF

General information: TITO was launched using: RESULT: Template: 2IQH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1451 -50971 -35.13 -164.95 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -35.13 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.192

(partial model without unconserved sides chains): PDB file : Tito_2IQH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2iqh-query.scw PDB file : Tito_Scwrl_2IQH.pdb: