Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSRTKNLLSHEDIQRLVKVNFGEEYEHKIGEIKELKGGMFNAIYLIQRVDEKNGIVLKVGVAPGRDLLTYEQDIMPTEVACYQLIKEK--TSIPIPEILKYDFSKKYIDSNYFFMEELKGVPLSK-ASKLMNKPSLDKIKSKLAEYLVQLHKIEGNYFGYFTDEKKYQYDTWKNAFLSMFGQLLEDCKFHKVNIPYERIEQALKYNIQFLEEIKKPSLVMFDCHEGNVFVNKRNGEYEIQGIIDFERAFWGDPAADFPAAFIMS---DDIRKEEAFLASYFEKSDTKKEYTRDDEIRFQMYRMYILTVMAGETFRYGPLYAKMQREWAKSGILKCLEILED 5IQC Chain:A ((7-301)) -----DNATNVKAMKYLIEHYFDNFK---VDSIEIIGSGYDSVAYLVN---N--EYIFKTKFSTN------KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYIS---DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID--NKQNVLEE-YILLRETIYNDLT----DIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGN---NRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNID------IEKAKEYQDIVEEYYPIETIVYGIKNIKQ---EFIENGRKEIYKR------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1310 -18898 -14.43 -65.39 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -14.43 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.317

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: