Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence YKCRISLMNGSDST----DDYADGKPGDTVNLEANNGDIVKVY--IRDNCYYDILNKEK--MPASYTIKT-- 1PWT Chain:? ((1-61)) -------MGTGKELVLALYDYQEKSPRE---VTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAY-VKKLD

General information: TITO was launched using: RESULT: Template: 1PWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 161 28795 178.85 564.61 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 178.85 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_1PWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1pwt-query.scw PDB file : Tito_Scwrl_1PWT.pdb: