Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------RMCILALAEKNQNPEKPSFLEDTFQEIEAPAAAMLSYGGHE----IHFELDKNCWPRLRHGSAVPSGYSAHVY---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 5ERX Chain:A ((22-574)) NPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRSGRIRLHVRIDERTAGYLAIGLAIGAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANYFGTQVRASISLGLAEDA--PERTSALNATWRSATCRVLAAATGARTANAGPVHFDIPLT--PPGRPAGK-PWTYTPPVTFDQPLDIDLSVDTVVISGHGAGVHPNLAALPTVAEPTAPRSGDNPLHPLALPLLRPQQVIMLGRPTLHRPVSVLLADAEVPVFALTTGPRWPDVSGNSQATGTRAVTTGAPRPAWLDRCAAMNRHAIAAVREQLAAHPLTTGLHVAAAVSHALRPGDQLVLGASNPVRDVALAGLDTRGIRVRSNRGVAGIDGTVSTAIGAALAYEGAHERTGSPDSPPRTIALIGDLTFVHDSSGLLIGPTEPIPRSLTIVVSNDNGGGDVSSRIFGTPHDVDVGALCRAYHVESRQIEVDELGPTLDQPGAGMRVLEVKADRSSLRQLHAAIKAAL

General information: TITO was launched using: RESULT: Template: 5ERX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 178 7278 40.89 113.72 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : 40.89 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_5ERX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5erx-query.scw PDB file : Tito_Scwrl_5ERX.pdb: