Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---SPGTLAEDADSPARPCKVT---ISMAVSPGGYFRQIYGEGLTTRGGRVAIE--NFKCTVDETCNNPSCPDVPPTWQVTSYD 2MYW Chain:A ((7-85)) SGRENLYFQGHMAAPARFCVYYDGHLPATRVLLMYVRIGTTATITARGHEFEVEAKDQNCKVILT-NGKQAPD---WLAAEPY-

General information: TITO was launched using: RESULT: Template: 2MYW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 201 1939 9.64 27.30 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 9.64 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_2MYW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2myw-query.scw PDB file : Tito_Scwrl_2MYW.pdb: