Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLGDNELTLTPI-PGKSGKAYMGSYPD-GKRIFVKMNTSP------------ILPGLAREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPY----DMNRKQIVNILTRLHRSRPLMTQLSRL-------GYAMETPVDLLQSWQETAPDALR---------------KNHFISEVMADLRQTIPGFREDHATIVHGDVRHSNWIETD--SGLIYLVDWDSVRLTDRMFDVAHMLCHYI----------SEHQWKEWLTYYGYKYN----QTVLSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR 2QG7 Chain:E ((109-432)) -----DSLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFFT--NG---RIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKR-ENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMH---------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 884 -1943 -2.20 -8.45 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain E : 0.65 3D Compatibility (PKB) : -2.20 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: