Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAENLSQMGAANVQRLVDQYGQQVIEKCPVS-------PVEYAFYERAAEELNSRGIKTPTLLSADPH-------PRRLRLEYIPGKVSQDE------VSGDDIL-KCLSHLHHTPPDPTWV--YHP---HSWPEKALEKSLAL--LRLPDRAAHQMRCFQQAGSV---LFRQKCLISGDTNAGNWGRREN-GEWVLFDWERFGTGSPAIDLAPLVKGMGSRQSILQLAERYSVHCPHMPARQLATEITLAKAWIVSEVMSLLYDRNKTDFELYLNWYRETLPGWLDEAISVL 5IGI Chain:A ((30-256)) -----NELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNR---LPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQ-KVADDVDRVRREFV-VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-EEGLAKLLLTYEAAGG--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 693 -287 -0.41 -1.48 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -0.41 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: