Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLRNSDRTREEILTRYFPQYRLIAPQAP-SGLGGASCIIEQ-GEQRLVLRQNHDPLASSSHFRRQFRALKR-LP-ADL-VPKPRFYRQGWMAVEYLAGEIGNTL---------------PETAVLAAMLCHLHRQPRLGWRV------T---LLPLLEQYWQQASAD-RRTPFW-LAQLKRLRKTGEPQPLRLSPLHMDVHAGNIVHTSL-GARLIDWEYAGDGDVALELAAVWSEDDAA-RQRLITDYARVAHMNADALRRQVRRWRPWVVMLMAGWFEMRFQQSRDKQFIALADDAWRQLQTKG 5UXD Chain:A ((4-299)) ---PEDLDALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDV-LERAAVEGRLLAMLAPHLDVAVPDWRISTSELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADD-GCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSAPSAIFEVALQAYAEGGGRPWPGLARH--CTEMFSAA-PLGYGLY-ALATGEAAHREAAAAALNPPEE--

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1183 -3863 -3.27 -14.69 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -3.27 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: