Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------MPSHLTHIWRLLKWGRTLARHGALTGIERDPLTPTPV--RRLVRVA-RLGARVPRQPRYADAFQSIG---------------------PAAIKLGQTLATRPDLVGEDAANDLLRLQDALPPVPFA-TIRAQMEQSFGRPLETLYSRFDEAPVGAASIAQ-------VHRAVTTDGRDVAVKVIRPGVIDQFNR-----DIQTYEWAAAHVEMLGGEVARLRPRLVIANMK----RWTAREL----DLRREAASASELGEAMEA------MPGYRVPAIDWDRTTGKVMTMEWIDGVKISDRDALIAAGHDVKEIAARLVNAFLRQAIAEGFFHA------DMHQGNLFVTANGDIVAIDFGIMGRIDRRARMWL-AEILYGLITGNY--RRVAEIHFEAQYVPG---HHNVEEFATALRAVG----EPMRGKPVRELSVGGMLDGLFAITRDFDMQTQP----------HLLLLQKTM----VMVEGVATALDPDINLWETSGPYVKEWLRAELGPEAKAADALIENVRILQRLPGLVKRIEEAFPEKGGAPPPPPLTEVRLIRVGSGWRYMLVALLAGLAGAGAMAAATTFL 4M59 Chain:A ((6-714)) PDAQVLVLAISSHPLPTLAAFLASRRDELLRADITSLLKALELSGHWEWALALLRWAGKEGAADASALEMVVRALGREGQHDAVCALLDETPLPPGSRLDVRAYTTVLHALSRAGRYERALELFAELRRQGVAPTLVTYNVVLDVYGRMGRSWPRIVALLDEMRAAGVEPDGFTASTVIAACSRDGLVDEAVAFFEDLKARGHAPSVVTYNALLQVFGKAGNYTEALRVLGEMEQNGCQPDAVTYNELAGTEEAARCLDTMASPNAFTYNTVMTAYGNVGKVDEALALFDQMKKTGFVPNVNTYNLVLGMLGKKSRFTVMLEMLGEMSRSGCTPNRVTWNTMLAVSGKRGMEDYVTRVLEGMRSSGVELSRDTYNTLIAAYGRCGSRTNAFKMYNEMTSAGFTPCITTYNALLNVLSRQGDWSTAQSIVSKMRTKGFKPNEQSYSLLLQCYAKGGNVAGIAAIENEVYGSGAVFPSWVILRTLVIANFKCRRLDGMETAFQEVKARGYNPDLVIFNSMLSIYAKNGMYSKATEVFDSIKRSGLSPDLITYNSLMDMYAKCSESWEAEKILNQLKCSQTMKPDVVSYNTVINGFCKQ-GLVKEAQRVLSEMVADGMAPCAVTYHTLVGGYSSLEMFSEAREVIGYMVQHGLK---PMELTYRRVVESYCRAFEEARGFLSEVKALEAYIEDA---

General information: TITO was launched using: RESULT: Template: 4M59.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2305 -60936 -26.44 -120.43 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -26.44 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.183

(partial model without unconserved sides chains): PDB file : Tito_4M59.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4m59-query.scw PDB file : Tito_Scwrl_4M59.pdb: