Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGAGAADATEDAATDATRGTDATRGTDATGGTDGPVPAARTAEEDRLVARVEELVGGR-VTAIERQ--PRWRKAWYLTVDRDGAELALYVRGDKQIDAEPYPGLDREAAILRILERN-GVPVPHVHGMSSD---PI----GIIMDRVPGTRDVAEAADDAQRRGIAEQYMEILARMHSIDVAEFAAAGIE--VPTTPEGAQLAFVDANERLYRRTKKAPEPLVEWALRWARRRLPTAGNRARFIHGDTG--QFLFVD-GRITCVYDFEASHIGDPLSDLAGLRTRAGTEPLGADIEHMIRHYQRVAGTTID--PSALSFYTATYMLTAVMALSGPLTELRPADQQAEYLTWDLMVRRAMLWAIAEVEGVKIEPAPPVTLPTGYPARVTTVLEGTVRRMVPATGVDEANQSAALALAQWAGAMVAVGMANVERDLDRAAVLLGHRPADQAAADAELERFVLAAGPEHDLALLEYFAAQTEARVAEAVPMRTRLEQYALPKVVL 5IGH Chain:A ((6-279)) -----------------------------------------TADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVD---DGRRWVLRIPRRAEV--SAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV-VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV----VNDKRLHRWQRWLDDDSS-WPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG---E-EGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYAL----------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1055 -12062 -11.43 -47.12 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -11.43 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: