Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------MLIDELTNDIDMYLGYSNLLDDKHKVIFLTESESNINFLIDVGLEKYIFRLSKNSQLGLKNQIKYEYDALK-----ILEKSYVTPRTFF-------LDDSTTFFDYGVLIMQYIKGRRLEYQRDFHEVAKTFGKIHSLNLDKIDVS---NFIVRDDI---IEDCL------EKSRINLKNFFKSPKIDADVKIKINDFLEWGEKNKKCKKF-----FEKDKWQVINNTEPHLD--------------------------------NFIISDRNR-KAYLLDWEKPVIADPSIELSYFLSPITTMWT------GDYAFSEDEKDDFF-------KTYIMYL-----DKRDRDIVERVK----------IYTPYICLKELSKLANRLWIYEHDTDINTDI----NEYKKLKEYINLE-----FMEYLTKNYNLY------- 5SWB Chain:A ((17-470)) KPVIKMYQIGDKPDNLDELLANANKIIEEKVG-AKLDIQYLGWGDYGKKMSVITSSGENYDIAF---ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQNFAFNGTLLAKY--GIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPVANGLPFVIDLEGDTTKVVNRYEVPRFKEHLK-TLHKFYEAGYIPKDVATSDTSFDLQQDTWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEKSMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNWILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTM--QQFDTAINTGTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKKLE

General information: TITO was launched using: RESULT: Template: 5SWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1581 105970 67.03 327.07 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 67.03 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_5SWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5swb-query.scw PDB file : Tito_Scwrl_5SWB.pdb: