Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPTPSPDTILFASHTTATAASVAQWVERHYDLP-VQQCYLIRRNLNDNYAVRAADGSRYVARLCAIRPRGPFNVDFEVALLAHLEAQGVGVASPVLAANGATSVALPFPEGPRVLVLFRHCEGSVPD--TPEDLHLTGATLAQIHSAAQSYTGPASRYVLDGHHLAGRTLDYLAAHPELAPSVLDAYRELIHRLLGELANV-EDTLTRVMCHGDTHGFNNHVYSDAEGARRAAFFDFDDAGPGFLAYDLCVWLWSNLPRKAPMEPDEALLQRWQQYLAGYRAGGGHVTDADLAALPLFVQLRHLWNMGEGVARIHH--WGANM---MSVDWIKKQLEVMAAWARLDLPA 1ZYL Chain:A ((6-315)) ------------FTFQTLHPDTIMDALFE-HGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAF-NGQTLLN----HQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIH-RPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD------GPMFVDLDDARNGPAVQDLWMLLNGDKA---------EQRMQLETIIEAYEEF-SEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAK-----

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1379 -47658 -34.56 -158.33 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -34.56 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: