Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELGNFQKSVDSDEIKTMAFEIIGEPVKAVSRICEQWGEAEDEVREYDVYVIETDA----------GKYTLKKTGS---------KEAQIYAQYLSKGEFHVPQYVGMRQAEDADWICMKYVEGNDMRDMT---DETTEKAAETLSKIQSYFWTPSMDK-AP-ENEVEQRFVEYWKRVLRRASS-------VADDPILRKAYQMFLD-----RQLTCPCTMSNGDFLQWNVIYDGENVVMIDWGFGGMMPYPLDIARFLAHATETRS--T-------FPFYMNDAQKELFLDRMYEALKTKISREQFNLDVKLATLNEYIEFVEAEEDEDGWYLEHAQALAEELLNL 3C5I Chain:D ((11-293)) --------LTDPLYIKKICLEKVPEWNHFTEDNLRVK--QIL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVDELYNTISEFEVYKTMSK-YKI-APQLLNTF----NGGRIEEWLYGDPLRIDDLKNPTILIGIANVLGKFHTLSRKRHLPEHWDRTPCIFKM-MEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTNKCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNY-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 868 11293 13.01 48.26 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain D : 0.63 3D Compatibility (PKB) : 13.01 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: