Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PCVITIDGPYNPKYNFYPSHGRGVTGPHGQVVI--GDFTCTVGATCNQLTCNAPQPEYSILGVDS- 1GOI Chain:A ((447-498)) ----------NLPIMTAPAYVPGTTYAQGALVSYQG----YVWQTKWGYITSAPGSDSAWLKVGRV

General information: TITO was launched using: RESULT: Template: 1GOI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 177 -660 -3.73 -13.46 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -3.73 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_1GOI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1goi-query.scw PDB file : Tito_Scwrl_1GOI.pdb: