Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAANPPRPSRSLQGKVAIVTGAGSRGETIGNGRAIALLLAEDGAKVVCVD-REEALAQRTAELVLAENEGASALAVAADVSSAEECARVVDAALRLFGRVDVLVNGVGVLGPPGTAVDVDAA-RWARGLEVNVTSMMLMAKYAVPAMRDNEPEAGVRGSIVNLGSVAGLRGGTPSLLYPTSKCAVVNMTRAMAAHHAKDGVRVNCVCPGMLYTPMVYDAEGGMSAEVREARRRRSLLQTEGNA-WDCAGPVRFLAGGEARWITGTVLTVDAGATCALAVSMPDGE 1HDC Chain:D ((3-254)) ------------LSGKTVIITG-GARGL----GAEAARQAVAAGARVVLADVLDEEGAATAREL------GDAARYQHLDVTIEEDWQRVVAYAREEFGSVDGLVNNAGI--STGMFLETESVERFRKVVEINLTGVFIGMKTVIPAMKD----AG-GGSIVNISSAAGLMGLALTSSYGASKWGVRGLSKLAAVELGTDRIRVNSVHPGMTYTPMT--AETGIRQ--GEGNYPNTPMGRVG-EPGEIAGAVVKLLSDTSSYVTGAELAVDGGWTTGPTVKYVMGQ

General information: TITO was launched using: RESULT: Template: 1HDC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1424 -53782 -37.77 -216.86 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -37.77 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_1HDC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1hdc-query.scw PDB file : Tito_Scwrl_1HDC.pdb: