Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence CVIGLTFNGNWVP----NNRFVISPG-GKFSFIANYVIIPVRLDESCHWHQDLAENAKPVIIPSGYGIKSC-------------------------- 3H8H Chain:A ((6-112)) SEIELVFRPHPTLMEKAQTRYIKTSGNATVDHLSKYLAVRLALEE-----LTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYA

General information: TITO was launched using: RESULT: Template: 3H8H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 147 -9023 -61.38 -147.91 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -61.38 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_3H8H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3h8h-query.scw PDB file : Tito_Scwrl_3H8H.pdb: