Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLLTILKKMKQKERELRLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATLLIFANKQDLPGALSSNAIREVLELD------------SIRSHHWCIQGCSAVTGENLLPGIDWLLDDISSRIFTAD 2FA9 Chain:B ((4-188)) ---SSVLQFLGLYKKTGKLVFLGLDNAGKTTLLHMLK--------PTLHPTSEELTIAGMTFTTFDLGGGIQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYI--------

General information: TITO was launched using: RESULT: Template: 2FA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 760 -92246 -121.38 -559.07 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -121.38 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2FA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2fa9-query.scw PDB file : Tito_Scwrl_2FA9.pdb: