Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEFQLDQEWRLRPI-KGDTGKTYIGMRD---------EDKVFIKRNTTP-----------MLAALSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPN---EIGQRNDVIDVLYHLHHSNSLKTMLTRIGGRVMTPEKMLQKYEKELPEELQK----------------NQFLQIVHRFLREQIPSIDKENLSVVHGDVNHRNWLVC-KNYLYLVDWDSIMFADPAVDIATILGNYV-------------------PLSSWNQWLISYGIRPTD-----------EMLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG 1NW1 Chain:B ((74-396)) -------EHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSERHLGPKLYGIF---S--GGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEP------DYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHIS-LSKSPVTFCHNDLQEGNILLPK--RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVE-----------------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 856 -23191 -27.09 -104.46 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -27.09 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: