Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISRLLSEQLGE-G-----EIELRNELPGGEVHAAWHLRYAG---------HDFFVKCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDA------HSAFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQ--LELAAEKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPD-------GPYIFDP-ACYWGDRECDLAMLPLH----------------------TEQPPQIYDGYQSVSPL-------------PADFLERQPVYQLYTLLNRARLFGGQHLVIAQQSLDRLLAA 5FTG Chain:A ((10-339)) --RRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG----EAMVLESVMFAILAE-RSLG-PKLYGIFPQ----GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPF-NKE-----PK---WLFGTMEKYLKEVLRIKFTEESRIKKLHKL--LSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHF------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 892 -6586 -7.38 -27.55 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -7.38 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: