Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHPAAQPTNQTSSHSPASPSNTEPCEQQSLKQQLLKQQPLKEQSLKQQSREQQLHTWLNEVFPNQS-LQHEKIPGDASFRSYHRLTVDNQHYIVMDAPPA---KESVKEFIAVGNLMA--GYVHVPKMIATDE------------QQGFIVLEDLGNTDFADVIAKDLTDAGELAKTARYYQQAMQEILAIQKIDINDAK-AVI--PSYDDALLRREMGLFSDWFLPYIGVKMTPDLETLWQDLQSDIIQQV--IAQPQVVVHRDFHSRNLMVLSH--SDELGVIDFQDAVIGAYTYDLASLLRDAYI-NYDENWVNTHLAHYHQLAQLDKSLAEFTVDFNIMSMQRHLKVLGIFVRLFERDGKDRYLVNLPKVFNDLLTCLKAISQWDKRPTFDKFADWMTAAAAPAFQQKIQG 6EF6 Chain:A ((27-322)) ---------------------------------------------------DVTIAQQALTHYDVSDNASLRLL-NLSENA-TYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESL-----------TLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRE--LPAADE-AMLPSFVFLRRLLLLAWMGSHT--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1215 -9070 -7.46 -33.97 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -7.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.227

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: