Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDQQQIIAVAEAVLTQRYGGQQHLTEPEELSGASGTVVLRLRVANNPFLSYRSVVVKYSPQTDDPIDDAAFLREVVAYQFTTSLSEEARPGPVMLGYDLEQHAIILTDSGDGDTLATLLENS--------DEEQRIRLLRNLGTSLGKMHAGTAGAELSFNTLLARMVRARDGAREIQAMRESMLENRILDGLEIVRRAGIDVPADVADIAGTVQVH-MLHGESRAFTPFDLSPDNIIFA-D-RT-QFLDYEWAGFRDVIFDVAGVIAGFPQYISARPISEAETKVFLEAWIAEVAGVWPALQNTETLHSRITAALVGWAFFSVSLLYLDGEEPRGTTRYGGGWIMADNGNYELVQDPEALEEDVERGASVLRSPAHTDFTAVEKAIRRDIGDAFSALAWYAAAGDTPRNAVIADFASHVAERLK 5UXA Chain:A ((6-256)) --------KQVIEIAK-KHNLFLKEETIQFNESGLDFQAVFAQDNN-----GIDWVLRLPRREDV---MPRTKVEKQALDLVNKYA-ISFQAPNWIIYT--EELIAYKKLD-GVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHT------PEEARMSMKQRMD-----A---VRAKF--GVGENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAF---------GEEGLEALILAYKEIGG---------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 946 -47379 -50.08 -198.24 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -50.08 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: