Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSASPSLQVTAFEGNRCIASGPLASVAEKIKEVLARGEAASVLVFDDQTGSQVDLNLRTLSEAAQGMPTPLIAPEPLESRNAGRPRLGVVAREVTLLPRQWEWLGQQQGGASATLRKLVDQARRENAGEEKIRAAREALYRFMTAMAGNAPGYEEALRALFAGDVHRFSILTSDWATDVRKHVGKLAPAAFGYAPSPLDVVIPLTKREAVLRAFGTAEVQSIERITNGASGAGVFKVKAN--QVDYLLRIEGPP-DGLRDPARQYACLKIAAEAGVAPRLVYADAESGVAVTDFIAPDSASVE-RSKADSLRRIVTAVRSLHDAPLFPEL---------------VDYLDGVDTLIRSCMETGIL-----------PKRAIETHRKFYGELAAAYPRKNLDLVSSHNDLNPGNVLFQK-ERVWLVDWESAFAADRYVDLAAIANFFT---------TEENEKELVLESYFGAALN---DLHRARFFLMQQANRMFYAMVVLNFVAAAEPAIKLTAAGMKGIRWSEVRGEMARINTTETKVRFAGALLNEALLAFQSPRFSQAVAQVKGSSKRRGA 2QG7 Chain:D ((110-433)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLEFQIINGG-T-NILIKVKDMSKQAKYLIRLYGPKTD---NREREKKISCILYNKNIAKKIYVFFTN--GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHS--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 913 -3685 -4.04 -15.42 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.59 3D Compatibility (PKB) : -4.04 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: