Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEELKDRIEDKYGSLRSLDSDSVGMAHDIFFLEFQDGKEMVL-KVGGEDLGFRFEREPYVLDWLADANLSVPEVVEFEINRGEEELSYLLMDKIEGQNINSYSEGRKFKFLSERAKE-KLVNESAKQLNSIHNSTSYPNFGAFRAKMEK-QYESNRWSETLLQILRKNELGGIEKGEFSHLHSKAESFLKENLSQLNTDSRP-VMVHQDFSFKNMIV----QGGEIRAVIDWERAISGHRELDLFKFERSISSKFRTKSIGEK-YGELLIQKYQSEHSLES-----GWEER-RNIYMLISLL------QTMWTYDDWSKNIPQKIRKKIKENMEKEFENRVSKRQTDIFQHME- 3CSV Chain:A ((4-333)) ------SREDEIRDFLATHGYADWNRTPRYQRLRSPTGAKAVLMDWSPEE-GGDTQPFVDLAQYLRNLDISAPEIYAEEHARG-----LLLIEDLGDALFTEVINN-------DPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTR-------LAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQ------LEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDV-PAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1398 43405 31.05 144.68 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 31.05 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.235

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: