Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGILEY-TSQLDIEFKKALGLIFKQELNLERIKQGEV-NYSYKVTSEQDIPLYHARIFRYEG-HPDTEKLLWISGELKKRNIPQARILYREKSSINFPNGFMVSNWIEGKNGWQSIKDGAYSLKNFTLETAKILLKIHEI-KVASFGWPYGKGWG---YYNTYQDRITGLPNNDEFKKLFNNDYLKRKYVLNIVSLLLKALEKIP-FPEQPILAHSDPTPDNIIVRNDRSLVLIDWDDSEGSW--WVRDYSYLRYWSDKPEEIKAGFFSGYGKIDMNESEIEIAELVEWILQSLRLLPYYKFSFADEGKYQKRMTRLKENVRQLKLLV 3JR1 Chain:A ((14-309)) NLYFQGMWKSISQVLAEQFGAYYFI--KHKEKLYSGEMNEIWLINDEVQTVFVKINERSYRSMFRAEADQLALLAKTNSINVPL-----VYGIGNSQGHSFLLLEALNKSK----------NKQSSFTIFAEKIAQLHQIQGPDKYGLDFDTWLGPIYQPNDWQTSWAKFFSENRIGWQLQICKEKGLIFGNIDLIVQIVADTLSKHNPKPSILHGNLWIENCIQVDDKIFVC----NPACYWGDRECDIAFSSLFEPFPTNFYQRYNEIYPLEEGYLERKLIYQLYYLLNFSYRY----------YNKKQSYVSLTQKLINQILHK-

General information: TITO was launched using: RESULT: Template: 3JR1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1419 20053 14.13 70.11 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 14.13 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.269

(partial model without unconserved sides chains): PDB file : Tito_3JR1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3jr1-query.scw PDB file : Tito_Scwrl_3JR1.pdb: