Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MTRSVHDALASHPVETTVHRRLHDVRPHEVFEVTY-DGQRVVC-KVAQHPLGDPELEARILQFVAAETPVPVPPIMAVGSDH-FVA-------GWCRDLPADPTVD-ETRSRAMGAGLARLHESASERFDAPGHLEAENGSLALDADDRWSDT-LVAFLDERRRFLEPRGYGDVATEVLGLVEAFPEAFDDVGDPT--LLHGNALPDHVGIDRSADPARVTRVIDFEHALVGPPAFDLLRSIGPTYGPPGATHDPDGRAAF-LEGYATVRPL-PADLEETFRPLGVV---NGVAYLRALHLQRGDRDAPQSVARRARGLAEHV-LEAASEVRSALRSE-------------- 3CSV Chain:A ((4-333)) SREDEIRDFLATHGYADWNRTPRY-------QRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNL-DISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDL-LIHLHDAQTPELARL--------------DPETLSEMTRLAFSE---YRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDV-----PAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1315 28119 21.38 97.97 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 21.38 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: