Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSYEFNVTQLRDLPFGDKNIKIITEKKGGWDSRFFILDGKVAVKVPRNITVQHSIIKEKIVTDLLRKELPLPIPDMNLAEIDSIDGNKIIIVIYEMLPGIELQNIHIIG---ENKIKTSKSLGLFLQKLHNIPDELAQILRSGISNVNNERNETDLELL-NSVLEYFEEMRNKTNVSSYIE-IMRTSMGSLKQMGTKIVPCHGDFMSNNILYNEIEGKISGIIDWGDFSFRNPIYDFAGLTY---EFGEEFLDSMLAAYASNTSPDFREKIVKLSRFVPFITVYFSRHMKKKIRFEKKYIKIELLESGRNQILHVR 3N4U Chain:A ((8-299)) ------QVEKAIEQLYPDFTINTIEISGEGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSE-TYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVL---DFREKINEDNKKIKKLL---SRELKG--PQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIRSIK----------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1248 -26438 -21.18 -93.09 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -21.18 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: