Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KNCKFNVLNGSGINVANGCCDVTKKQVTVSPFTVSCTESCGLAIVSGG-------- 1KBE Chain:A ((1-49)) GSVTHRFSTKSWLSQV---CNVCQKSMI---FGVKCKH-CRLKCHNKCTKEAPACR

General information: TITO was launched using: RESULT: Template: 1KBE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 117 -11038 -94.34 -269.21 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -94.34 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_1KBE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1kbe-query.scw PDB file : Tito_Scwrl_1KBE.pdb: