Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------NG-----CLVKILDGHRSLGERCIEFGQ------EGYVTVHAEKRGYRYSLVNVQTGADCKDKGEGTNLQVVP- 1H8L Chain:A ((302-380)) GIWGFVLDATDGRGILNATISV-ADINH-PVTTYKDGDYWRLLVQGTYKVTASARGYDPVTKTVEVDSK---GGVQVNFTLSRT

General information: TITO was launched using: RESULT: Template: 1H8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 210 22166 105.55 388.88 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 105.55 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.610

(partial model without unconserved sides chains): PDB file : Tito_1H8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1h8l-query.scw PDB file : Tito_Scwrl_1H8L.pdb: