Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------EQDCYYTV-----WTSNGNSWLRSIRDKTRSDHTLGIHGASVYFDEDCYPHYHSKE 2BBY Chain:? ((175-243)) RARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIH-KNTWELKPEYRHYQ---

General information: TITO was launched using: RESULT: Template: 2BBY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 106 939 8.86 19.98 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 8.86 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.104

(partial model without unconserved sides chains): PDB file : Tito_2BBY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2bby-query.scw PDB file : Tito_Scwrl_2BBY.pdb: